Absence of Runx3 expression in normal gastrointestinal epithelium calls into question its tumour suppressor function
نویسندگان
چکیده
The Runx3 transcription factor regulates cell fate decisions during embryonic development and in adults. It was previously reported that Runx3 is strongly expressed in embryonic and adult gastrointestinal tract (GIT) epithelium (Ep) and that its loss causes gastric cancer. More than 280 publications have based their research on these findings and concluded that Runx3 is indeed a tumour suppressor (TS). In stark contrast, using various measures, we found that Runx3 expression is undetectable in GIT Ep. Employing a variety of biochemical and genetic techniques, including analysis of Runx3-GFP and R26LacZ/Runx3(Cre) or R26tdTomato/Runx3(Cre) reporter strains, we readily detected Runx3 in GIT-embedded leukocytes, dorsal root ganglia, skeletal elements and hair follicles. However, none of these approaches revealed detectable Runx3 levels in GIT Ep. Moreover, our analysis of the original Runx3(LacZ/LacZ) mice used in the previously reported study failed to reproduce the GIT expression of Runx3. The lack of evidence for Runx3 expression in normal GIT Ep creates a serious challenge to the published data and undermines the notion that Runx3 is a TS involved in cancer pathogenesis.
منابع مشابه
Absence of Runx3 expression in normal gastrointestinal epithelium calls into question its tumor suppressor functio
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Author reply to: RUNX3 is expressed in the epithelium of the gastrointestinal tract
The article by Levanon et al (2011) reports that expression of the transcription factor Runx3 is undetectable in gastrointestinal (GIT) epithelium. These results contradict our results, particularly those in Li et al (2002), which indicate that Runx3 is strongly expressed and functions as tumour suppressor in GIT. Here, I aim to clarify the issues by summarizing the findings associated with thi...
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عنوان ژورنال:
دوره 3 شماره
صفحات -
تاریخ انتشار 2011